Journal: iScience

Article Title: Mission SpaceX CRS-19 RRRM-1 space flight induced skin genomic plasticity via an epigenetic trigger

doi: 10.1016/j.isci.2024.111382

Figure Lengend Snippet:

Article Snippet: OCT-embedded tissue was cryosectioned (10 μm), fixed with cold acetone, blocked with 10% normal goat serum (NGS), and incubated with specific primary antibodies against anti-5-mC (#28692S, Cell Signaling Technology; 1:200 dilution), anti-5-hmC (#ab214728, abcam; 1:200 dilution), E-cadherin (#13–-1900, Invitrogen; 1:400 dilution), E-cadherin (#13-1900, Invitrogen; 1:400 dilution) CD31 (#550274, BD Pharmingen; 1:200 dilution), F4/80 (#MCA497R, Bio-Rad; 1:200 dilution); ARAF (#22129-1-AP, Proteintech Group, Inc.; 1:100 dilution) followed by appropriate fluorescence conjugated secondary antibodies (Alexa 568-tagged α-sheep, #A-21099, Invitrogen, 1:200 dilution; Alexa 568-tagged α-rat, # ab175476, Abcam, 1:200 dilution; Alexa 488-tagged α-rabbit, #A-11008, Invitrogen, 1:200 dilution).

Techniques: Methylation, Enzyme-linked Immunosorbent Assay, Isolation, Methylation Sequencing, Software, Microscopy, Fluorescence

Journal: Frontiers in Endocrinology

Article Title: PKC-mediated phosphorylation and activation of the MEK/ERK pathway as a mechanism of acquired trastuzumab resistance in HER2-positive breast cancer

doi: 10.3389/fendo.2022.1010092

Figure Lengend Snippet: Selected antibodies, fluorescence intensities and Log 2 FC in protein & phosphoprotein quantities in T15 and JIMT-1 relative to SKBR3.

Article Snippet: MEK/ERK , A-Raf - phosphoTyr301/Tyr302_68kDa , Biorbyt , orb5910 , Rb pAb , 21540 , 22850 , 19923 , -0.09 , -0.20.

Techniques: Fluorescence